Lazio hope to keep their perfect Europa League record going with a difficult trip to FC Twente, giving Samuel Gigot his debut with Boulaye Dia and Pedro.
It kicks off at the Grolsch Veste Stadium in Enschede at 20.00 UK time (19.00 GMT).
The Biancocelesti won both opening fixtures 3-0 over Dynamo Kyiv and 4-1 against OGC Nice, which put them top of the Europa League table.
They suffered a 1-0 defeat away to Juventus at the weekend, ending a run of four straight wins in all competition.
Tijjani Noslin is suspended, with Matteo Guendouzi and Manuel Lazzari injured, so Gigot finally gets his debut in defence.
Loum Tchaouna, Pedro and Mattia Zaccagni fan out behind centre-forward Boulaye Dia, resting Taty Castellanos.
Michal Sadilek and Younes Taha are out of action, but FC Twente have had a decent start to the Europa League, holding both Manchester United and Jose Mourinho’s Fenerbahce to 1-1 draws.
Former Atalanta and Glasgow Rangers striker Sam Lammers leads the way.
The Dutch team are unbeaten in their last 12 competitive home matches.
Twente vs. Lazio line-ups
Twente: Unnerstall; Van Rooij, Hilgers, Bruns, Salah-Eddine; Vlap, Regeer; Van Wolfswinkel, Steijn, Van Bergen; Lammers
Lazio: Mandas; Marusic, Gigot, Romagnoli, Pellegrini; Vecino, Dele-Bashiru; Tchaouna, Pedro, Zaccagni; Dia
Ref: Dabanovic (MNE)
